MET Dimerization CHO
CBP74470
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| I. Background | |
| C-Met 屬于受體酪氨酸激酶家族，是一種異二聚體，由高度糖基化的細(xì)胞外 50 kDa α 鏈和跨膜 140 kDa β鏈組成，通過二硫鍵連接在一起。作為一種天然的 HGF 受體，它表達(dá) 于各種上皮細(xì)胞的表面，并促進(jìn)腫瘤的發(fā)生。與 HGF 結(jié)合后，兩個(gè)亞基二聚化，導(dǎo)致兩個(gè) 酪氨酸殘基（Tyr1234 和 Tyr1235 ）自磷酸化。酪氨酸殘基的自磷酸化導(dǎo)致一個(gè)激活的多功 能停靠位點(diǎn)，該位點(diǎn)由 SH2 結(jié)構(gòu)域、PTB 結(jié)構(gòu)域和 Met 結(jié)合結(jié)構(gòu)域 （MDB） 組成，該結(jié) 構(gòu)域募集接頭分子。 | |
| II. Description | |
MET Dimerization CHO 報(bào)告基因藥靶模型很好的模擬了體內(nèi) MET 的信號(hào)轉(zhuǎn)導(dǎo)過程， 原理見下圖所示。 Figure 1. MET Dimerization CHO 細(xì)胞模型原理圖 |
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| III. Introduction | |
| Expressed gene: | MET |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12K+10%FBS+5μg/ml Puromycin+5 μg/ml Blasticidin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Representative Data | |
 Figure 2. Recombinant MET Dimerization CHO stably expressing MET.  Figure 3. Dose Response of Recombinant Human HGF in Met Dimerization CHO(C19).
 Figure 4. Inhibition of Recombinant Human HGF induced Met Dimerization by Met Abs in Met Dimerization CHO(C19). |
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