PDGF/PDGFRB Effector Reporter Cell
CBP74316
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產(chǎn)品描述
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| I. Background | |
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血小板衍生生長因子（PDGF）是一類重要的生長因子，主要由血小板、內(nèi)皮細(xì)胞和 巨噬細(xì)胞分泌，在細(xì)胞增殖、遷移、分化和血管生成等生物學(xué)過程中發(fā)揮關(guān)鍵作用。它不 僅在正常生理過程中（如傷口愈合和組織修復(fù)）起重要作用，還在多種病理過程中（如癌 癥、纖維化和動脈粥樣硬化）扮演重要角色。PDGF 家族包括 PDGF-A、PDGF-B、PDGF- C 和 PDGF-D 四種亞型。PDGF 通過與其受體（PDGFR）結(jié)合，激活下游信號通路，調(diào)控 細(xì)胞行為。 血小板衍生生長因子受 0 體α（PDGFRA），又稱為 CD140A 或 PDGFR2，是一種細(xì)胞 表面酪氨酸激酶受體，屬于血小板衍生生長因子家族。 |
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| II. Description | |
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PDGF/PDGFRB Effector Reporter Cell 報告基因藥靶模型很好的模擬了體內(nèi) PDGFRB 的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
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| III. Introduction | |
| Expressed gene: | PDGF/PDGFRB |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+200 μg/ml Hygromycin B+2 μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
IV. Representative Data Figure 2. Recombinant PDGF/PDGFRB Effector Reporter Cell stably expressing PDGFRB.  Figure 3. Dose Response of Recombinant Human PDGF-BB in PDGF/PDGFRB Effector Reporter Cell(C26).  Figure 4. Inhibition of Human PDGF-BB-induced Reporter Activity by PDGFRB Blocking Abs in PDGF/DGFRB Effector Reporter Cel(C26). Inhibition of Human PDGF-BB-induced Reporter Activity by PDGFB Neutralization Ab in PDGF/PDGFRB Effector Reporter Cell(C26). |
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