CCR8/β-Arrestin/CHO
CBP71363
| I. Background | |
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趨化因子(C-C motif)受體 8 (CCR8)是一種主要表達于調(diào)節(jié)性 T 細(xì)胞(Treg)上的趨化因子 受體，屬于 G 蛋白偶聯(lián)受體。CCR8 的配體有 CCL1、CCL8、CCL16 和 CCL18。 |
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| II. Description | |
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CCR8 β-Arrestin CHO 報告基因藥靶模型很好的模擬了體內(nèi) CCR8/β-Arrestin 的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。當(dāng)缺乏配體刺激時，β-Arrestin 不與 CCR8 結(jié)合，融合β-Arrestin的熒光素酶處于失活構(gòu)象，當(dāng) CCR8 遭遇配體刺激時，融合熒光素酶報告基因的β-Arrestin被招募，使熒光素酶報告基因處于激活狀態(tài)，加入其底物后發(fā)光信號增強。
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| II. Introduction | |
| Host Cell: |
CHO |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12k+10%FBS+5μg/ml puromycin+5μg/ml blasticidin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): |
Functional assay for CCR8 |
| Transducer: | β-Arrestin |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
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Figure 3. Dose response of Human Recombinant CCL1 in CCR8 β-Arrestin CHO(C6). |
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Figure 4. Inhibition of rhCCL1-induced β-Arrestin Recruitment in CCR8 β-Arrestin CHO(C6). |
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