C3aR/β-Arrestin/CHO
CBP71386
| I. Background | |
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補體過敏毒素 C3a 是補體系統(tǒng)激活（經典、凝集素或旁路途徑）過程中，由 C3 轉化 酶裂解 C3 蛋白α鏈釋放的小分子多肽（77 個氨基酸，分子量 9 kDa）。作為補體級聯的核 心炎癥介質，C3a 在血漿中以前體形式（無活性） 存在，經羧肽酶 N 切除 C 端精氨酸后轉 化為活性形式 C3a-desArg（失去受體結合能力但保留部分免疫調節(jié)功能）。 |
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| II. Description | |
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C3aR β-Arrestin CHO 報告基因藥靶模型很好的模擬了體內 C3aR/β-Arrestin 的信號轉導過程，原理見下圖所示。
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| II. Introduction | |
| Host Cell: |
CHO |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | F12k+10%FBS+5μg/ml Puromycin+5μg/ml Blasticidin |
| Mycoplasma Status: | Negative |
| Storage: | Liquid nitrogen immediately upon delivery |
| Application(s): | Functional assay for C3aR receptor |
| Transducer： | β-Arrestin |
| Ⅲ. Description of Host Cell Line | |
| Organism: | Hamster |
| Tissue: | Ovary |
| Morphology: | Epithelial |
| Growth Properties: | Adherent |
| Ⅳ. Representative Data | |
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Figure 2. Recombinant C3aR/β-Arrestin/CHO constitutively expressing C3aR/β-Arrestin. |
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Figure 3. Dose Response of Human Recombinant CCL1 in C3aR β-Arrestin CHO(C8). |
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