hFAP/MC-38
CBPG0099
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| I. Background | |
| 成纖維細(xì)胞活化蛋白-α（FAP）是一種細(xì)胞表面 II 型跨膜糖蛋白絲氨酸蛋白酶，作用 于多種激素和細(xì)胞外基質(zhì)(ECM)成分。該蛋白參與許多細(xì)胞過程，包括組織重塑、傷口愈 合、炎癥、纖維化和腫瘤生長。這種非典型絲氨酸蛋白酶屬于后脯氨酸切割酶 S9b 家族， 具有二肽基肽酶和內(nèi)肽酶活性。 FAP 單體具有 5 個(gè)潛在的 N -糖基化位點(diǎn)、13 個(gè)半胱氨酸殘基、3 個(gè)與絲氨酸蛋白酶高 度保守的催化結(jié)構(gòu)域相對應(yīng)的片段、一個(gè)疏水跨膜片段和一個(gè)由 6 個(gè)氨基酸組成的胞質(zhì)短 尾。FAP 單體無活性，活化的 FAP 異二聚體與 α3β1 整合素或 uPAR 可調(diào)控多種下游信號 通路，從而對表達(dá) FAP 的細(xì)胞和微環(huán)境產(chǎn)生不同的功能影響。這些涉及的信號通路包括 PI3K/AKT、RAS/ERK、音猬因子 (SHH) 和粘著斑激酶 (FAK) 通路。 |
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| II. Introduction | |
| Host Cell: | MC-38 |
| Expressed gene: | FAP |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: | DMEM+10%FBS+5μg/ml Puromycin |
| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| III. Description of Host Cell Line | |
| Organism: | Mus musculus, mouse |
| Tissue: | Colon |
| Morphology: | Epitheloid cell |
| Growth Properties: | Adherent |
| IV. Representative Data | |
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Figure 1. Recombinant hFAP/MC-38 stably expressing FAP. |
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| V. In VIVO Data | |
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Index |
Content |
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Animal |
C57BL/6J, 7weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
1×10^6/0.1ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hFAP/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3. hFAP/MC38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
