4ig-B7H3(CD276)/MC-38
CBPG0022
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產(chǎn)品描述
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| I. Background | |||
| 免疫調(diào)節(jié)蛋白的 B7 家族是結(jié)構(gòu)相關(guān)的細(xì)胞表面蛋白，在抗原呈遞細(xì)胞/腫瘤細(xì)胞內(nèi)和 腫瘤/免疫細(xì)胞之間的信號(hào)傳導(dǎo)中起著核心作用。B7 家族成員（包括 CTLA-4、PD-1 和 PD-L1）之間的相互作用介導(dǎo)的免疫檢查點(diǎn)機(jī)制可以產(chǎn)生致瘤和共調(diào)節(jié)功能。B7-H3 蛋白 表達(dá)與許多實(shí)體瘤（包括宮頸癌、卵巢癌和乳腺癌等）的不良預(yù)后具有相關(guān)性。B7-H3 （CD276）有兩種異構(gòu)體（2Ig 和 4Ig），沒有確定的同源受體，B7-H3 在多種惡性腫瘤中 均有表達(dá)，與惡性腫瘤的生長(zhǎng)、轉(zhuǎn)移、復(fù)發(fā)、預(yù)后不良等因素密切相關(guān)。B7-H3 可下調(diào)Ｔ 輔助 1 型介導(dǎo)的免疫反應(yīng)抑制 CD4+T 細(xì)胞活化，并抑制細(xì)胞因子的產(chǎn)生，發(fā)揮促進(jìn)癌細(xì)胞 免疫逃逸的作用。 | |||
| II. Introduction | |||
| Host Cell: | MC-38 | ||
| Expressed gene: | 4ig-B7H3 | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | DMEM+10%FBS+ 2 μg/ml Puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mus musculus, mouse | ||
| Tissue: | Colon | ||
| Morphology: | Epitheloid cell | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | ||
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Figure 1. Recombinant 4ig-B7H3/MC38 stably expressing 4ig-B7H3. |
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| V. In VIVO Data | ||
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Index |
Content |
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Animal |
C57BL/6J, 7weeks, female |
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Implanted Position |
Subcutaneous (right flank region ) |
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Injected Cell Numbers |
1×10^6/0.1ml |
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Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. 4ig-B7H3(CD276)/MC-38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.4ig-B7H3(CD276)/MC-38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
