hPSMA/MC-38
CBPG0046
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產(chǎn)品描述
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| I. Background | |||
| PSMA 蛋白也稱為谷氨酸羧基肽酶 II 和葉酸水解酶 1，它是一種跨膜糖蛋白酶，存在 于細胞表面。PSMA 主要在前列腺、腎臟、小腸以及中樞和外周神經(jīng)系統(tǒng)中表達。PSMA 在正常前列腺組織和非前列腺癌組織（如淚腺、神經(jīng)系統(tǒng)、十二指腸）中表達水平很低， 但在前列腺癌組織中的表達比正常組織高 100-1000 倍。在前列腺癌中，PSMA 表現(xiàn)出羧肽 酶活性，可以促進葉酸釋放谷氨酸，并激活谷氨酸系統(tǒng)，調(diào)節(jié)鈣離子通道。此外，它能夠 將細胞生存信號從絲裂原激活蛋白激酶途徑轉(zhuǎn)向磷酸肌苷-3 激酶/Akt 致癌途徑，最終促進 腫瘤細胞生長。PSMA 在其他實體腫瘤中也有表達，比如胃癌、胰腺癌、乳腺癌、肺癌等， 但是在這些腫瘤中，PSMA 分布于腫瘤血管而不是腫瘤細胞。在非前列腺癌中，PSMA 主 要參與腫瘤血管的生成，PSMA 可促進血管粘連蛋白肽的下游 MMP-2 的激活。 | |||
| II. Introduction | |||
| Host Cell: | MC-38 | ||
| Expressed gene: | hPSMA | ||
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) | ||
| Freeze Medium: | 90% FBS+10% DMSO | ||
| Culture Medium: | DMEM+10%FBS+2 μg/ml Puromycin | ||
| Mycoplasma Testing: | Negative | ||
| Storage: | Liquid nitrogen | ||
| III. Description of Host Cell Line | |||
| Organism: | Mus musculus, mouse | ||
| Tissue: | Colon | ||
| Morphology: | Epitheloid cell | ||
| Growth Properties: | Adherent | ||
| IV. Representative Data | ||
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Figure 1. Recombinant hPSMA/MC-38 stably expressing PSMA. |
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| V. In VIVO Data | ||
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Index |
Content |
|
Animal |
C57BL/6J, 6weeks, female |
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Implanted Position |
Subcutaneous (right lower flank region ) |
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Injected Cell Numbers |
1×10^6/0.1ml |
|
Matrix gel |
Without matrix gel |
1. Weight Change
Figure 2. hPSMA/MC38 Mean Body Weight ± SEM.
2. Tumor Size
Figure 3.hPSMA/MC38 Mean Tumor Volume ± SEM .
NOTE：The above data was provided by Cobioer (cell data) and Truway Bio (in vivo data model).
